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KMID : 1177720160100010023
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Journal of Alternatives to Animal Experiments
2016 Volume.10 No. 1 p.23 ~ p.28
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The Application of Good Laboratory Practice (GLP) on in Vitro Skin Irritation Test
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Lee Won-Hee
Kim Nam-Soo
Kim Bo-Ra
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Abstract
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Various guidelines of alternative test have been published following increased interest in alternative test. Among these guidelines, in vitro skin irritation test is composed of applying a test substance on an artificial human skin model and determining skin irritation measured by cell viability assay using MTT test method. To perform this test to become objectively and reliably, we built all the test procedures following Good Laboratory Practice (GLP). This paper introduces the in vitro skin irritation test method using a human skin model and describes the process how researcher have been through to comply with GLP.
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KEYWORD
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Skin irritation, Reconstructed human epidermis, GLP
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